Effect of extraction solvents on triterpenoid contents and quantification of saponins Rb1, Rb3, and Rd in Rubus cochinchinensis using high-performance liquid chromatography
Vol. 135 No. 1A (2026), Original Article
Vol. 135 No. 1A (2026)

Effect of extraction solvents on triterpenoid contents and quantification of saponins Rb1, Rb3, and Rd in Rubus cochinchinensis using high-performance liquid chromatography

Original Article
https://doi.org/10.26459/hueunijns.v135i1A.7900
Published 2026-03-17
Man Nguyen Quang
Faculty of Basic Sciences, University of Medicine and Pharmacy, Hue University, Hue, Vietnam
Lan Huong Hoang Thi
Hue City Department of Health, Hue, Vietnam
Dung Bui Tien
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Minh Hang Hoang Thi
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Minh Tran Thanh
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Phu Nguyen Vinh
Faculty of Basic Sciences, University of Medicine and Pharmacy, Hue University, Hue, Vietnam
My Linh Le Thi
Faculty of Basic Sciences, University of Medicine and Pharmacy, Hue University, Hue, Vietnam
Khanh Ly Vo Thi
Faculty of Pharmacy, Hue Medical College, Hue, Vietnam
Hong Hanh Nguyen Thi
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Hieu Le Trung
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
Le Lam Son*
Faculty of Chemistry, University of Sciences, Hue University, Hue, Vietnam
PDF (Vietnamese)

Keywords

Ngấy hương
tổng triterepnoid
Rb1
Rb3
Rd Rubus cochinchinensis
total triterpenoids

How to Cite

1.
Nguyễn Quang M, Hoàng Thị LH, Bùi Tiến D, Hoàng Thị MH, Trần Thanh M, Nguyễn Vĩnh P, Lê Thị ML, Võ Thị KL, Nguyễn Thị HH, Lê Trung H, Lê Lâm S. Effect of extraction solvents on triterpenoid contents and quantification of saponins Rb1, Rb3, and Rd in Rubus cochinchinensis using high-performance liquid chromatography. hueuni-jns [Internet]. 2026Mar.17 [cited 2026May19];135(1A):51-8. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7900
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Abstract

In this study, we evaluated the effect of extraction solvents on the total triterpenoid content and quantified three selected triterpenoid saponins Rb1, Rb3, and Rd in the extract of Rubus cochinchinensis. The total triterpenoid content was determined by using a colorimetric method with vanillin reagent in a perchloric acid medium, and the saponin compounds were quantified by means of high-performance liquid chromatography. Seven solvent systems were investigated, among which ethanol yielded the highest extraction efficiency for the triterpenoids (56.40 ± 0.53 mg oleanolic acid/g). The content of Rb1, Rb3, and Rd was 327.58 ± 2.84, 324.95 ± 7.91, and 135.17 ± 1.09 μg/g, respectively. Compared with well-known medicinal plants such as Korean Red Ginseng and Panax quinquefolius, R. cochinchinensis demonstrated superior levels of triterpenoid saponins. These findings suggest that R. cochinchinensis is a promising natural source for the development of biologically active products, particularly in the fields of pharmaceuticals and functional foods.

https://doi.org/10.26459/hueunijns.v135i1A.7900
PDF (Vietnamese)

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