Keywords
polyphenol
Helicteres hirsuta Lour.
thân cây An xoa optimisation
polyphenols
Helicteres Hirsuta Lour.
stem of Helicteres hirsuta
How to Cite
Abstract
This study optimised the extraction process of polyphenols from the stems of Helicteres hirsuta Lour. with the Response Surface method. Key factors affecting the extraction efficiency, namely ethanol concentration, solvent-to-material ratio, temperature, and extraction time, were investigated through single-factor experiments and a Box-Behnken design. The results indicate that the total polyphenol content (TPC) was significantly influenced by ethanol concentration, temperature, and especially extraction time. A quadratic regression model was developed and found to be statistically significant (p < 0.0001) with good predictive ability (adjusted R² = 0.9357). The optimal conditions were determined as 76.10% ethanol, a 19.36 mL/g solvent-to-material ratio, an extraction temperature of 62.28 °C, and an extraction time of 89.50 minutes, yielding a predicted TPC of 45.10 mg GAE/g extract. The experimental validation showed good agreement with the predicted values, confirming the reliability of the model. This study highlights the potential of H. hirsuta stems as a valuable source of polyphenols for pharmaceutical and functional food applications.
References
- Pham HNT, Van Vuong Q, Bowyer M, Scarlett C, editors. Phytochemical profiles and potential health benefits of Helicteres hirsuta Lour. Proc 1st Int Electron Conf Food Sci Funct Foods; 2020.
- Cutrim CS, Cortez MAS. A review on polyphenols: Classification, beneficial effects and their application in dairy products. International Juornal Dairy Tecnology. 2018;71(3):564-78.
- Erukainure OL, Mesaik AM, Muhammad A, Chukwuma CI, Manhas N, Singh P, et al. Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity. Biomedicine & Pharmacotherapy. 2016;83:1478-84.
- Kammerer DR, Kammerer J, Valet R, Carle R. Recovery of polyphenols from the by-products of plant food processing and application as valuable food ingredients. Food Research International. 2014;65:2-12.
- Sajadimajd S, Bahramsoltani R, Iranpanah A, Patra JK, Das G, Gouda S, et al. Advances on natural polyphenols as anticancer agents for skin cancer. Pharmacological research. 2020;151:104584.
- Long D, Le V, Quang H, Han H, Hue T, Trang N. Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue. Indian Journal of Science and Technology. 2023;16(44):4114-22.
- Pham NK, Bauerova K, Cuong NM. Studies on hepatoprotective effects of Vietnamese medicinal plants. Vietnam Journal of Science and Technology. 2023;61(5):725-50.
- Le TH, Nguyen MN, Nguyen THC, Le LS, Ho XAV, Tran TM, et al. Hepatoprotective and Antioxidant Activities of Methanolic Helicteres hirsuta Leaf Extract. Chemistry & Biodiversity. 2025;22(12):e00878.
- Bộ Y tế. Dược điển Việt Nam V. Hà Nội: Nhà xuất bản Y học; 2017.
- Ministry of Science Technology. Determination of substances characteristic of green and black tea - Part 1: Content of total polyphenols in tea - Colorimetric method using Folin-Ciocalteu reagent. Vietnam: Ministry of Science and Technology; 2013.
- Miller JN, Miller JC. Statistics and Chemometrics for Analytical Chemistry. 6th ed. Harlow: Pearson Education; 2010.
- Nacif de Abreu I, Mazzafera P. Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiology and Biochemistry. 2005;43(3):241-8.
- Ćujić N, Šavikin K, Janković T, Pljevljakušić D, Zdunić G, Ibrić S. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry. 2016;194:135-42.
- Pinelo M, Rubilar M, Jerez M, Sineiro J, Núñez MJ. Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of agricultural and food chemistry. 2005;53(6):2111-7.
- Nguyen VT, Bowyer MC, Van Altena IA, Scarlett CJ. Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity. Chemical Papers. 2016;70(6):713-25.
- Minh T, Koóng. Kỹ thuật sản xuất dược phẩm. Hà Nội: Nhà xuất bản Y học; 2007.
- Al-Farsi MA, Lee CY. Optimization of phenolics and dietary fibre extraction from date seeds. Food Chemistry. 2008;108(3):977-85.
- Wang J, Sun B, Cao Y, Tian Y, Li X. Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chemistry. 2008;106(2):804-10.
- Naczk M, Shahidi F. Extraction and analysis of phenolics in food. Journal of Chromatography A. 2004;1054(1-2):95-111.
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