Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170
Vol. 130 No. 1A (2021), Original Article
Vol. 130 No. 1A (2021)

Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170

Original Article
https://doi.org/10.26459/hueunijns.v130i1A.5785
Published 2021-03-10
Nguyen Thi Anh Thu
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Ho Thi Trang
University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
PDF (Vietnamese)

Keywords

Bacillus subtilis
nattokinase
nat05
expression Bacillus subtilis
nattokinase
nat05
biểu hiện

How to Cite

1.
Thư NTA, Trang HT. Expression of nat05 encoding for a nattokinase in Bacillus subtilis BD170. hueuni-jns [Internet]. 2021Mar.10 [cited 2024Dec.27];130(1A):69-75. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5785
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Abstract

Nattokinase, a serine protease found in the Japanese Natto product, can specifically dissolve fibrin fibers causing coagulation and is useful in endogenous thrombolysis. Currently, nattokinase is produced with the help of traditional fermentation and recombinant DNA technology approaches. In this study, we presents the optimization of time and the inducing substance (IPTG) concentration of gene nat05 encoding for a nattokinase in Bacillus subtilis BD170 with strong protease activity. After 10 hours’ cultivation, nat05 expresses 9.5 times higher than the control. At the IPTG concentration of 2 mM, nat05 exhibits the strongest activity, which is 10 times higher than the control.

https://doi.org/10.26459/hueunijns.v130i1A.5785
PDF (Vietnamese)

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