Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures
Vol. 129 No. 1A (2020), Hue University Journal of Science: Natural Science
Vol. 129 No. 1A (2020)

Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures

Hue University Journal of Science: Natural Science
https://doi.org/10.26459/hueunijns.v129i1A.5676
Published 2020-03-20
Pham Thi My Tram
Thu Dau Mot University, 6 Tran Van On St., Thu Dau Mot, Binh Duong, Vietnam
Ngo Ke Suong
Institute of Tropical Biology, Ho Chi Minh City, 9/621 Hanoi Road., Dist. Thu Duc, Ho Chi Minh City, Vietnam
Le Thi Thuy Tien
VNUHCM – University of Technology, 268 Ly Thuong Kiet St., Dist. 10, Ho Chi Minh City, Vietnam
PDF (Vietnamese)

Keywords

callus
cell suspension cultures
Ehretia asperula Zoll. et Mor.
xa den Ehretia asperula Zoll. et Mor.
huyền phù tế bào
mô sẹo
xạ đen

How to Cite

1.
Mỹ Trâm PT, Sương NK, Thủy Tiên LT. Effects of medium composition on Xa den (Ehretia asperula Zoll. et Mor.) cell cultures. hueuni-jns [Internet]. 2020Mar.20 [cited 2024Nov.22];129(1A):31-4. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/5676
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Xa den (Ehretia asperula Zoll. et Mor.) belongs to the Boraginaceae family and appears in Southeast Asian countries. Xa den is often used to treat diseases such as tumors, emphysema, and detoxification. The effects of plant growth regulators and carbon sources on the formation and proliferation of callus from Xa den leaves in vitro were studied. The results show that callus proliferates well on the medium supplemented with 30 mg/L glucose, 0.4 mg/L 2,4-D, and 0.1 mg/L BA. In this medium, the callus is yellowish-green, friable, and rapid proliferating. After the first subculture, the callus is milky white and friable after 10 weeks of culture. This callus is used as a material for cell suspension cultures. Suspension cells grow in the dark better than in the light with the highest biomass after four weeks of culture (149.933 g/L).

https://doi.org/10.26459/hueunijns.v129i1A.5676
PDF (Vietnamese)

References

  1. Pant B. Application of plant cell and tissue culture for the production of phytochemicals in medicinal plants. Advances in Experimental Medicine and Biology. 2014;808:25-39.
  2. Subedi T. Phytochemical studies of Taxus species and their uses in cancer treatment. Janapriya Journal of Interdisciplinary Studies. 2017;6:160-71.
  3. Ramani S, Jayabaskaran C. Enhanced catharanthine and vindoline production in suspension cultures of Catharanthus roseus by ultraviolet-B light . Journal of Molecular Signaling. 2008;3(9):1-6.
  4. Hoa HQ, Khánh TC. Đặc điểm thực vật của ba loại cây thuốc thuộc chi Cườm rụng (Ehretia P. BR.), họ Vòi voi (Boraginaceae). Tạp chí Dược liệu. 2009;14(3):137-41.
  5. Kuo YH, Kuo LMY. Antitumour and anti_AIDS triterpenes from Celastrus hindsii. Phytochemistry. 1997;44(7):1275-81.
  6. Ly TN, Shimoyamada M, Yamauchi R. Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity. Agricultural and Food Chemistry. 2006; 54(11):3786-93.
  7. Cường NH. Nghiên cứu thành phần hóa học và thăm dò hoạt tính sinh học cây xạ đen (Celastrus hindsii Benth. and Hook.) và cây cùm rụm răng (Ehretia dentata Courch.) [Luận án]. [Hà Nội: VN]: Viện Khoa học và Công nghệ Việt Nam; 2008.
  8. Ly TN. Separation process of rosmarinic acid and their derivatives from Celastrus hindsii Benth leaves. Journal of Science and Technology. 2016;54(2C):380-7.
  9. Trâm TTM, Hương TT, Loan LQ, Dũng NH, Tuấn TT. Khảo sát sự sinh trưởng, khả năng kháng oxy hoá và hàm lượng phenolic của cây xạ đen Ehretia asperula Zollinger et Moritzi in vitro dưới tác động của đèn led. Tạp chí Khoa học công nghệ và Thực phẩm. 2018;16(1):38-48.
  10. Anh TNT, Thu NTB. Nghiên cứu nhân nhanh cây xạ đen Ehretia asperula Zoll. et Mor. Tạp chí Dược liệu. 2012;17(4):244-8.
  11. Tien LTT, Minh TV. Tissue cultures of xa den Ehretia asperula Zollinger et Moritzi. An Giang University Journal of Science. 2015;3(3):113-23.
  12. Tuan TT, Loan NTK, Thuy PTT, Hang NTT, Trang NTH, et al. Quanlitative rosmarinic acid content in ex vitro plant and initial micropropagation of Celastrus hindsii. Vietnamese Journal of Biotechnology, 2016;14(1A): 283-90.
  13. LTT Hong, Minh TV. Saponin accumulation in cell suspension culture of Ehretia asperula Zollinger et Moritzi. In: 8th International Conference on Advances in Civil, Structural and Environmental Engineering - ACSEE; 2019 January 12–13; Kuala Lumpur, Malaysia. New York (USA): Institute of Research Engineers and Doctors; 2019. p. 21-25.
  14. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research. 1986;50:151-8.
  15. Sahraroo A, Babalar M, Mirjalili MH, Fattahi Moghaddam MR, Nejad Ebrahimi S. In vitro callus induction and rosmarinic acid quantification in callus culture of Satureja khuzistanica Jamzad (Lamiaceae). Iranian Journal of Pharmaceutical Research. 2014;13(4):1447-1456.
  16. Cương LK, Chiến HX, Nam NB, Hương TT, Nhựt DT. Ảnh hưởng của một số yếu tố lên khả năng tăng sinh mô sẹo "xốp" và bước đầu nuôi cấy huyền phù tế bào sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.). Tạp chí Sinh học. 2012;34(3):265-276.
  17. Lượng NĐ, Tiên LTT. Công nghệ tế bào. Hồ Chí Minh: Nhà xuất bản Đại học Quốc gia; 2006. tr.376.
  18. Benítez-García I, EmilioVanegas-Espinoza P, Meléndez-Martínez AJ, Heredia FJ, Paredes-López, O, Del Villar-Martínez AA. Callus culture development of two varieties of Tagetes erecta and carotenoid production. Electronic Journal of Biotechnology. 2014;17(3):107-13.
  19. Phua QY, Chin CK, Asri ZRM, Lam DYA. The callugenic effects of 2,4-Dichlorophenoxy acetic acid (2,4-D) on leaf explants of sabah snake grass (Clinacanthus nutans). Pakistan Journal of Botany. 2016;48(2):561-566.
  20. André SB, Mongomaké K, Modeste KK, Edmond KK, Tchoa K, Hilaire KT, Justin KY. Effects of plant growth regulators and carbohydrates on callus induction and proliferation from leaf explant of Lippia multiflora Moldenke (Verbenacea). International Journal of Agriculture and Crop Sciences. 2015;8(2):118-27.
  21. Ilczuk A, Jagieo-Kubiec K, Jacygrad E. The effect of carbon source in culture medium on micropropagation of common ninebark (Physocarpus opulifolius (L.) Maxim.) ‘Diable D’or’. Acta Scientiarum Polonorum Hortorum Cultus. 2013;12(3):23-33.
  22. Trejgell A, Jarkiewicz M, Tretyn A. The effect of carbon source on callus induction and regeneration ability in Pharbitis nil. Acta Physiologiae Plantarum. 2006;28(6):619-26.
  23. Sumaryono, Muslihatin W, Ratnatnadewwi D. Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb.) plantlets. Hayati Journal of Biosciences. 2012;19(2):88-92
  24. Yan YH, Li JL, Zhang XQ, Yang WY, Ma YM, Zhu YQ, et al. Effect of naphthalene acetic acid on adventitious root development and associated physiological changes in stem cutting of Hemarthria compressa. PLoS One. 2014;9(3):1-6.
  25. Bhagya N, Chandrashekar KR. Effect of growth regulators on callus induction from Cyclea peltata (lam.) Hook. f. Thoms. Asian Journal of Pharmaceutical and Clinical Research. 2013;6(4):85-88.
  26. Nhân NH, Quảng HT, Lộc NH. Ảnh hưởng của môi trường nuôi cấy lên khả năng sinh trưởng của callus cây bách bệnh (Eurycoma longifolia Jack). Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên. 2019;128(1E):69-76.
  27. Yeoman MM, Davidson AW. Effect of light on cell division in developing callus cultures. Annals of Botany. 1971;35:1085-100.
  28. Tiên LTT, Việt BT, Lượng NĐ. Khảo sát vài yếu tố ảnh hưởng đến sự sinh tổng hợp taxol của các hệ thống tế bào Taxuz Wallichiana Zucc. in vitro. Tạp chí phát triển khoa học và công nghệ. 2010;13(3):67-77.
  29. Mir MY, Kamili AN, Hassan OP, Tyub S. Effect of light and dark conditions on biomass accumulation and secondary metabolite production in suspension cultures of Artemisia amygdalina Decne. Journal of Himalayan Ecology and Sustainable Development. 2017;12:107-112.
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright (c) 2020 Array