Cloning genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector pMYV719 for genetic transformation
Vol. 131 No. 1C (2022), Original Article
Vol. 131 No. 1C (2022)

Cloning genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector pMYV719 for genetic transformation

Original Article
https://doi.org/10.26459/hueunijns.v131i1C.6667
Published 2022-09-30
Phung Thi Bich Hoa
Faculty of Biology, University of Education, Hue University, 34 Le Loi St., Hue, Vietnam
Nguyen Hoang Tue
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Pham Thi Huyen Trang
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Tran Gia Cat Tuong
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Huynh Thi Huyen Trang
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
Nguyen Xuan Huy
Departmentof Science, Technology and International Relations, Hue University, 03 Le Loi St., Hue, Vietnam
Nguyen Hoang Loc*
Faculty of Biology, University of Sciences, Hue University, 77 Nguyen Hue St., Hue, Vietnam
PDF (Vietnamese)

Keywords

Từ khóa: Chitinase 42 kDa, Chi42, syncodChi42-1, syncodChi42-2, Trichoderma asperellum. Keywords: Chitinase 42 kDa, Chi42, syncodChi42-1, syncodChi42-2, Trichoderma asperellum.

How to Cite

1.
Phùng TBH, Nguyễn HT, Phạm THT, Trần GCT, Huỳnh THT, Nguyễn XH, Nguyễn HL. Cloning genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector pMYV719 for genetic transformation. hueuni-jns [Internet]. 2022Sep.30 [cited 2024Nov.25];131(1C):55-62. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6667
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Abstract

In this study, chitinase genes containing a signal peptide sequence, such as Chi42, syncodChi42-1 and syncodChi42-2, were cloned in the plant expression vector pMYV719 and successfully transferred into Agrobacterium tumefaciens LBA 4404. Among them, Chi42 is a wild-type gene of Trichoderma asperellum SH16. Both genes syncodChi42-1 and syncodChi42-2 are derived from Chi42, which was optimized for codon usage for plant expression. Agrobacterium bacteria-harbouring pMYV719/chitinase vector was used for genetic transformation into peanuts (Arachis hypogaea L.) to enhance resistance to phytopathogenic fungi in further studies.

https://doi.org/10.26459/hueunijns.v131i1C.6667
PDF (Vietnamese)

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