Compatibility and optimization of antigen content to develop combined acellular diphtheria-tetanus-pertussis (DTaP) vaccine at Institute of Vaccines and Medical Biologicals
Vol. 134 No. 1C (2025), Original Article
Vol. 134 No. 1C (2025)

Compatibility and optimization of antigen content to develop combined acellular diphtheria-tetanus-pertussis (DTaP) vaccine at Institute of Vaccines and Medical Biologicals

Original Article
https://doi.org/10.26459/hueunijns.v134i1C.7755
Published 2025-06-17
Nguyen Thi Thu Hoa*
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Xuan Khanh Ha Nguyen
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Van Duoc Nguyen
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Ngoc Nhon Tran
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Thi Lan Anh Pham
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Thi Bich Trang Luong
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Thi Lan Phuong Nguyen
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
Huu Thai Duong
Institute of Vaccines and Medical Biologicals, 9 Pasteur, Nha Trang City, Khanh Hoa Province, Viet Nam
PDF (Vietnamese)

Keywords

compatibility
optimized DTaP formulation
aluminum hydroxide adjuvant
IVAC tính tương thích
tối ưu công thức DTaP
tá chất nhôm hydroxide
IVAC

How to Cite

1.
Nguyễn TTH, Nguyễn XKH, Nguyễn V Được, Trần NN, Phạm TLA, Lương TBT, Nguyễn TLP, Dương HT. Compatibility and optimization of antigen content to develop combined acellular diphtheria-tetanus-pertussis (DTaP) vaccine at Institute of Vaccines and Medical Biologicals. hueuni-jns [Internet]. 2025Jun.17 [cited 2025Oct.31];134(1C):5-19. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/7755
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Abstract

Compatibility between components and the amount of antigens in combination vaccines are important factors determining the safety, efficacy, and stability of that vaccine. To develop the diphtheria-tetanus-acellular pertussis (DTaP) vaccine at the Institute of Vaccines and Medical Biologicals (IVAC), we evaluated the compatibility among the components and optimized the amount of antigens in the vaccine formulation. The antigen components, including tetanus antigen (T), diphtheria antigen (D), and recombinant pertussis toxoid (rPT), together with filamentous haemagglutination factor (FHA), and aluminum hydroxide adjuvant, were evaluated for compatibility by using physicochemical and immunological methods. Design Expert version 12 software was used to optimize the amount of each antigen in the DTaP vaccine formulation. The results show that the components are compatible with each other. The optimal DTaP vaccine formula contains 40 Lf/mL D, 20 Lf/mL T, 10 µg/mL rPT, 20 µg/mL FHA, and 0.6 mg Al3+/mL respectively, corresponding to the diphtheria potency of 94,47 IU/mL, tetanus potency of 107,86 IU/mL, and pertussis potency of 9,65 IU/mL. The DTaP vaccine developed by IVAC meets the standard of the Vietnamese Pharmacopoeia V, 2018, of the Ministry of Health, and WHO.

https://doi.org/10.26459/hueunijns.v134i1C.7755
PDF (Vietnamese)

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