Effects of culture medium on callus growth of Gynostemma pentaphyllum (Thunb.) Makino – a valuable medicinal plant
Vol. 130 No. 1C (2021), Original Article
Vol. 130 No. 1C (2021)

Effects of culture medium on callus growth of Gynostemma pentaphyllum (Thunb.) Makino – a valuable medicinal plant

Original Article
https://doi.org/10.26459/hueunijns.v130i1C.6364
Published 2021-09-30
Hoang Tan Quang*
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Thi Diem Thi
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Tran Thuy Lan
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Pham Mai Thu Thuy
Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue, Vietnam
Vu Duc Hoang
University of Education, The University of Da Nang, 459 Ton Duc Thang St., Lien Chieu, Da Nang, Vietnam
Thi A Kim Phan
Department of Sciences and Technology, 54 Hung Vuong St., Tam Ky City, Quang Nam, Vietnam
PDF (Vietnamese)

Keywords

callus
chất điều hòa sinh trưởng
Gynostemma pentaphyllum
gypenoside
HPLC callus
Gynostemma pentaphyllum
gypenoside
HPLC
plant growth regulator

How to Cite

1.
Hoàng TQ, Phạm TDT, Lan TT, Phạm MTT, Vũ Đức H, Phan T Á K. Effects of culture medium on callus growth of Gynostemma pentaphyllum (Thunb.) Makino – a valuable medicinal plant. hueuni-jns [Internet]. 2021Sep.30 [cited 2024Nov.24];130(1C):127-3. Available from: https://jos.hueuni.edu.vn/index.php/hujos-ns/article/view/6364
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Abstract

Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan) is a traditional medicinal herb belonging to the Cucurbitaceae family. G. pentaphyllum grows widely in Southern China, Japan, Korea, and Vietnam. The essential bioactive components of Jiaogulan are saponin glycosides (gypenosides) and antioxidants. Jiaogulan exhibits bioactive activities such as anticancer, antioxidant, cholesterol-reducing agent, immunopotentiation, and others. In this study, the primary callus of Jiaogulan was used as a material to evaluate the influence of the culture medium on the induction and growth of secondary calli. The results reveal that the callus cultured on the MS medium supplemented with 2.0 mg/L kinetin and 0.5 mg/L indole-3-butyric acid has the best growth ability, high rate of secondary callus induction (100%), and good callus quality for suspension culture. The concentration of gypenoside and Rb1 in callus is 36.298 and 0.009 mg/g dry weight. The gypenoside concentration of callus is lower than that of leaves (65.58%) and almost similar to that of stems (92.38%) from natural samples. The suitable solvent for the extraction of gypenoside is methanol. The obtained callus will be used as material for cell suspension culture in further studies.

https://doi.org/10.26459/hueunijns.v130i1C.6364
PDF (Vietnamese)

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